Generation of iPSC line HEL24.3 from human neonatal foreskin fibroblasts
نویسندگان
چکیده
منابع مشابه
Expression of TGF-β3 in Isolated Fibroblasts from Foreskin
Background: The multifunctional transforming growth factor beta (TGF-β) is a glycoprotein that exists in three isoforms. TGF-β3 expression increases in fetal wound healing and reduces fibronectin and collagen I and III deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. Fibroblasts are key ...
متن کاملGeneration and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts.
Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of M...
متن کاملexpression of tgf-β3 in isolated fibroblasts from foreskin
background: the multifunctional transforming growth factor beta (tgf-β) is a glycoprotein that exists in three isoforms. tgf-β3 expression increases in fetal wound healing and reduces fibronectin and collagen i and iii deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. fibroblasts are key cells in t...
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Induced pluripotent stem cells (iPSCs) are becoming mainstream tools to study mechanisms of development and disease. They have a broad range of applications in understanding disease processes, in vitro testing of novel therapies, and potential utility in regenerative medicine. Although the techniques for generating iPSCs are becoming more straightforward, scientists can expend considerable reso...
متن کاملGrowth and Isolation of Human Cytomegalovirus on a New Human Fetal Foreskin Fibroblast-derived Cell Line in Iran
Cell culture technique has been used for detection and confirmation of many different viruses in clinical samples. Although, new diagnostic methods have been developed for viral infections, traditional cell culturetechnique is still regarded as the “gold standard” for several infectious agents such as human cytomegalovirus(HCMV). In the present study, a new human fetal foreskin fibr...
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ژورنال
عنوان ژورنال: Stem Cell Research
سال: 2015
ISSN: 1873-5061
DOI: 10.1016/j.scr.2015.05.012